Cloned (Comment) | Organism |
---|---|
glutathione S-transferase-fusion protein expressed in Escherichia coli, expressed in HEK293-cell, eGFP-fusion protein expressed in HEK293-cell | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C110S/C112S | all cysteine residues of the regulatory domain mutated | Mus musculus |
C110S/C112S/C162S/C234S/C248S/C296S | six N-terminal-most cysteines mutated | Mus musculus |
C110S/C112S/C162S/C234S/C248S/C296S/C315S/C355S/C365S/C394S/C404S/C408S/C476S | all 13 cysteine residues mutated | Mus musculus |
C110S/C162S/C234S/C248S/C296S/C355S/C365S/C394S/C404S/C408S/C476S | mutant protein containing a single cysteine residue within the regulatory domain (Cys112) and within the catalytic core domain (Cys315) | Mus musculus |
C162S/C234S/C248S/C296S/C315S/C355S/C365S/C394S/C404S/C408S/C476S | all cysteine residues of the catalytic core domain mutated | Mus musculus |
C315S/C355S/C365S/C394S/C404S/C408S/C476S | seven C-terminal-most cysteines mutated | Mus musculus |
C476S | residue of the tetramerization domain | Mus musculus |
DELTA-C-terminal 24 residues | removed tetramerization domain | Mus musculus |
DELTA1-40 | simulates tyrosine hydroxylase 1 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | oxidation inhibits activity and leads to the formation of high molecular weight aggregates in a dithiothreitol-reversible manner, only cysteine-less mutants are resistant to this effect | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
4 * 56000, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified and cleaved from its fusion tag | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin | - |
Mus musculus | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 56000, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
tryptophan hydroxylase 2 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
metabolism | rate-limiting enzyme in the serotonin biosynthesis | Mus musculus |